Additional file 2: of Biofilm production by Haemophilus influenzae and Streptococcus pneumoniae isolated from the nasopharynx of children with acute otitis media

Supplementary Data S2: Different cut-offs used to determine the biofilm production by H. influenzae: the different cut-offs used were as followed where AB represented the stained wells containing attached bacteria, CW the stained control wells containing bacteria-free medium only and G the bacterial growth control i) AB – CW: strong production > 0.30, moderate production 0.10–0.30, negative 6, moderate production 2–6, negative 1.10, moderate production 0.35–1.10, negative

补充数据S2：用于测定流感嗜血杆菌（H. influenzae）生物膜生成能力的不同临界值：所用临界值规则如下，其中AB指代染色后的含附着细菌的孔，CW指代仅含无菌培养基的染色对照孔，G指代细菌生长对照。i) AB – CW：强生成>0.30，中等生成0.10–0.30，阴性6，中等生成2–6，阴性1.10，中等生成0.35–1.10，阴性