Microbial and environmental dataset from Crocker Reef, Florida Keys, 2014-2015

Crocker Reef was the site of an integrated reefscape characterization effort focused on calcification and related biogeochemical processes as part of the USGS Coral Reef Ecosystem Study (CREST) project. This effort included two intensive seasonal sampling trips to capture summer (July 8 to 17, 2014) and winter (January 29 to February 5, 2015) conditions. This data release represents water column microbial and environmental data collected for use as metadata in future publications examining reef metabolic processes via metagenomes derived from water samples and fine-scale temporal and spatial carbonate chemistry measurements. Microbial data are total bacterial counts per milliliter seawater, total viral counts per milliliter of seawater, and plate counts using Thiosulfate Citrate Bile Salts Sucrose (TCBS) agar of Vibrio (bacteria) per milliliter of seawater. Environmental data are water temperature, salinity, dissolved oxygen, and pH.

克罗克礁（Crocker Reef）是美国地质调查局（USGS）珊瑚礁生态系统研究（CREST）项目中，针对钙化作用及相关生物地球化学过程开展一体化礁体景观特征研究的实施区域。该研究实施了两次高强度季节性采样航次，分别获取了2014年7月8日至17日的夏季环境与2015年1月29日至2月5日的冬季环境数据。本次数据发布涵盖水体微生物与环境实测数据，这些数据将作为元数据，用于后续通过水样宏基因组（metagenomes）及精细时空尺度碳酸盐化学测量开展珊瑚礁代谢过程研究的学术出版物中。其中微生物数据包括：每毫升海水的总细菌计数、每毫升海水的总病毒计数，以及采用硫代硫酸盐柠檬酸盐胆盐蔗糖（Thiosulfate Citrate Bile Salts Sucrose, TCBS）琼脂培养基进行的每毫升海水中弧菌（Vibrio）属细菌的平板菌落计数。环境数据则涵盖水温、盐度、溶解氧及pH值。