Transcriptome analysis of rice plants overexpressing OsMYBS1

To investigate the genes affected by OsMYBS1, we performed transcriptome sequencing analysis using the overexpression line, which exhibited the highest expression levels, and compared it with WT plants. For RNA sequencing, flag leaves from both Hejiang19 and the overexpression line OE-1 at the heading stage were collected, with each sample comprising a pool of 10 plants. Each rice variety included three biological replicates.为探究受OsMYBS1调控的基因，我们选取表达量最高的过表达株系开展转录组测序分析，并将其与野生型（Wild Type, WT）植株进行对照比较。在RNA测序实验中，我们于抽穗期采集合江19与过表达株系OE-1的剑叶样本，每份样本由10株植株混合而成。每个供试水稻材料均设置3次生物学重复。