Single cell RNA-sequencing of cardiac CD45+ populations in mouse with transverse aortic constriction (TAC)

It is already well known that the immune cells called macrophages are involved in the mechanisms of heart failure (HF). Macrophages are divised into several subtypes based on behavior.Some subsets are highly inflammatory, while some support tissue repair. Recent studies have suggested that the classical M1/M2 classification is not suitable for some pathological processes of the heart. Single cell sequencing (scSeq) is an ideal tool to clarify the macrophage heterogeneity in HF. It provided a higher resolution of cellular differences and a better understanding of individual cell function. In this study, we aimed to identify a macrophage subset that linked with heart failure, and furtherly screen the target and investigate the mechanism. CD45+ cells from heart of mice with/without TAC (6 per group) were sorted and the mRNA profiles of single cells were investagted by 10x Genomics technology