Single cell RNA-sequencing of cardiac CD45+ populations in mouse with transverse aortic constriction (TAC)

It is already well known that the immune cells called macrophages are involved in the mechanisms of heart failure (HF). Macrophages are divised into several subtypes based on behavior.Some subsets are highly inflammatory, while some support tissue repair. Recent studies have suggested that the classical M1/M2 classification is not suitable for some pathological processes of the heart. Single cell sequencing (scSeq) is an ideal tool to clarify the macrophage heterogeneity in HF. It provided a higher resolution of cellular differences and a better understanding of individual cell function. In this study, we aimed to identify a macrophage subset that linked with heart failure, and furtherly screen the target and investigate the mechanism. CD45+ cells from heart of mice with/without TAC (6 per group) were sorted and the mRNA profiles of single cells were investagted by 10x Genomics technology现已明确，被称为巨噬细胞（macrophages）的免疫细胞参与心力衰竭（heart failure, HF）的发病机制。巨噬细胞可根据功能行为分为多种亚型：部分亚型具有高度促炎特性，而另一些则参与组织修复过程。近期研究表明，经典的M1/M2分型并不适用于心脏的部分病理生理过程。单细胞测序（single cell sequencing, scSeq）是阐明心力衰竭中巨噬细胞异质性的理想工具，该技术可实现更高分辨率的细胞差异解析，并帮助研究者更深入地理解单个细胞的功能特性。本研究旨在筛选出与心力衰竭相关的巨噬细胞亚型，并进一步筛选潜在靶点、探究其发病机制。我们对经主动脉弓缩窄（Transverse Aortic Constriction, TAC）处理及假手术处理的小鼠心脏组织中提取的CD45阳性细胞（每组6只）进行了分选，并通过10x Genomics技术检测了单细胞的mRNA表达谱。