scRNA_seq_CoronaVac.cloupe

CoronaVac, a promising inactivated SARS-CoV-2 vaccine, may induce gene expression changes in peripheral blood mononuclear cells (PBMC) at early stages of vaccination. We used single cell RNA sequencing (scRNA-seq) to analyze the PBMC transcriptomics of CoronaVac-vaccinated participants (the vaccination group, two individuals, various timepoints at 0, 14, 28, 35 days) and COVID-19-recovered patients (the recovery group, three individuals, 8 months after recovery). The h5 file is the merged-barcode-feature-matrix file processed by the Cell Ranger (version 5).The cloupe file can be viewed using the Loupe Browser (version 5).

克尔来福（CoronaVac）作为一款颇具应用前景的SARS-CoV-2灭活疫苗，可在疫苗接种早期诱导外周血单个核细胞（peripheral blood mononuclear cells，PBMC）发生基因表达改变。本研究采用单细胞RNA测序（single cell RNA sequencing，scRNA-seq）技术，对接种克尔来福疫苗的受试者（疫苗组，共2名个体，分别于接种后0、14、28、35天多个时间点采样）以及新冠康复患者（康复组，共3名个体，于康复后8个月采样）的PBMC转录组进行分析。本数据集包含的h5格式文件为经Cell Ranger（版本5）处理后的合并条形码-特征矩阵文件；cloupe格式文件可通过Loupe Browser（版本5）进行浏览查看。