Ipsilateral brain injuries observed histologically from individual aging mice.

Ipsilateral brain injury was recognized through hypochromic staining when examined at 24–48 hours post-HI or cystic infarctions at 4–5 weeks post HI. Column 1: Animal ID of each aging mouse examined. Columns 2–6: injured brain structures indicated by ‘+’. Abbreviations: HIPP—hippocampus; LAT CORT—lateral cortex; STR—striatum; TH—thalamus; MB/BST—midbrain and brainstem areas. Column 7: ipsilateral regions with hypochromic staining where clearly recognized boundaries were present and quantifiable in 6 animals. These regions were measured at 8 coronal levels (bregma 1.9, 1.2, 0.5, -0.2, -1.1, -1.5, -2.4 and -3.2 mm respectively) and normalized as a % of total ipsilateral hemispheric area. The mean±SE from multiple coronal levels were presented for each animal. Column 8: ipsilateral brain injury observed for other animals in which injury margins were difficult to demarcate. For these animals, the coronal levels at which hypochromic staining or infarctions were observed were indicated for each animal instead.Ipsilateral brain injuries observed histologically from individual aging mice.