Whole transcriptome sequencing of splenic influenza-specific CD8+ T cells in wt and Notch1/2ko mice. Whole transcriptome sequencing of splenic influenza-specific CD8+ T cells in wt and Notch1/2ko mice

To more specifically determine whether the TEC program is controlled by Notch, we generated molecular definitions of TECs and MPCs by whole transcriptome sequencing of sorted KLRG1-CD127+ and KLRG1+CD127- H-2 Db-NP specific CD8+ T cell populations from wild-type mice. These definitions were used to analyze data derived from RNAseq of splenic H-2 Db-NP+CD8+ T cells from WT and Notch1-2-KO mice 10 days post-infection with A/HKx31.

为更精准地确定终末效应CD8+ T细胞（Terminal Effector CD8+ T Cells, TECs）程序是否受Notch调控，我们从野生型（Wild Type, WT）小鼠中分选得到H-2 Db限制性核蛋白（Nucleoprotein, NP）特异性CD8+ T细胞群中的杀伤细胞凝集素样受体G1阴性白细胞介素7受体α阳性（KLRG1⁻CD127⁺）与杀伤细胞凝集素样受体G1阳性白细胞介素7受体α阴性（KLRG1⁺CD127⁻）细胞亚群，通过全转录组测序（whole transcriptome sequencing）构建了TECs与记忆前体细胞（Memory Precursor Cells, MPCs）的分子特征定义。我们利用上述分子特征定义，对感染A/HKx31毒株后第10天的野生型与Notch1-2双基因敲除小鼠的脾脏H-2 Db-NP⁺CD8+ T细胞的RNA测序（RNA sequencing, RNA-seq）数据进行分析。