Quartet of familiar males – 16p11.2 Del mouse strain – 2 WT + 2 Del/+ - M2
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We monitored the individual and social behaviours of each quartet of mice over three days and nights in the Live Mouse Tracker system (LMT, plugin 931; de Chaumont et al. 2019 Nat. Biomed. Engin.). This system tracks individually mice living in a group over several days and nights and extracts automatically the number, total duration and mean duration of more than thirty behavioural events describing the posture of the mouse, the types of social contacts, the dynamic social approach and escapes and complex social groupings (see de Chaumont et al. 2019 Nat. Biomed. Engin.). In this system, the four mice (10-14 weeks of age; 2 WT mice and 2 Del/+ mice) from the same housing cage (housed together from weaning on) were left undisturbed for 71 hours in a large transparent Plexiglas cage (50 x 50 x 40 cm), with fresh bedding, a house (width: 100 mm, depth: 75 mm, height: 40 mm) in red Plexiglas, 6 dental cotton rolls as well as food and water ad libitum. Light/dark cycle and temperature conditions were similar to those of the housing room (12/12h light/dark, lights on at 07:00 AM, 100 lux when the lights were on). Each recording session started between 03:00 and 04:00 PM. At the end of the session, mice were placed back in their home cage and the LMT setup was cleaned with soap water and dried with paper towels. The upload includes the sqlite database from LMT (processed).
本研究借助活体小鼠追踪系统(Live Mouse Tracker,简称LMT,插件版本931;de Chaumont等,2019,《自然-生物医学工程(Nature Biomedical Engineering)》),对每一组四只小鼠的个体行为与社会行为开展了为期三昼夜的监测。 该系统可对群居饲养的小鼠进行多昼夜的个体追踪,并自动提取三十余种行为事件的发生次数、总持续时长与平均持续时长,涵盖小鼠体态、社会接触类型、动态社交趋近与逃离行为,以及复杂社交群体互动等维度(详见de Chaumont等,2019,《自然-生物医学工程(Nature Biomedical Engineering)》)。 本次实验中,取自同一饲养笼(自断奶起便群居饲养)的4只10~14周龄小鼠(2只野生型(Wild Type, WT)小鼠与2只Del/+基因型小鼠)被置于尺寸为50×50×40 cm的透明有机玻璃(Plexiglas)饲养笼中,且不受干扰地饲养71小时。笼内配备新鲜垫料、红色有机玻璃制作的躲避屋(宽100 mm、深75 mm、高40 mm)、6支牙科棉卷,以及不限量的食物与自由饮水。 实验环境的光照周期与温度均与原饲养室保持一致:采用12小时光照、12小时黑暗的循环模式,照明于每日07:00开启,光照强度为100勒克斯。 每一轮记录均于当日15:00至16:00之间启动。 单轮实验结束后,小鼠被放回原饲养笼,随后使用肥皂水清洁LMT实验装置,并用纸巾擦干。 本次上传内容包含经预处理的LMT系统生成的SQLite数据库文件。



