Regulation of Liver metabolism by ALKBH1. Regulation of Liver metabolism by ALKBH1

AlkB homolog 1 (ALKBH1) has been reported to act as a DNA 6mA demethylase to remove 6mA modificatione in ukaryotic genomes. Our previous data showed downregalted ALKBH1 in mouse and human fatty liver and increased DNA 6mA levels in mouse fatty liver. We hypothesize that ALKBH1 may be involved in hepatic lipid metabolism. To test this hypothesis, we generated liver-specific ALKBH1 knockout (Alkbh1 f/f, Albumine-cre; AKO) mice and used high-throughput RNA sequence and other assays to directly study the role of ALKBH1 during the development of hepatic steatosis. Overall design: Examination of differential expressed genes between control (Alkbh1 f/f ) and AKO hepatocytes.据报道，AlkB同源蛋白1（AlkB homolog 1, ALKBH1）是一种DNA 6mA去甲基化酶，可在真核生物基因组中去除6mA甲基化修饰。我们此前的研究数据显示，在小鼠和人类的脂肪肝组织中ALKBH1的表达量下调，而小鼠脂肪肝组织中的DNA 6mA水平则有所升高。据此我们提出假说：ALKBH1可能参与肝脏脂质代谢过程。为验证该假说，我们构建了肝脏特异性ALKBH1敲除（Alkbh1 f/f, 白蛋白-cre; AKO）小鼠模型，并通过高通量RNA测序及其他实验手段，直接探究ALKBH1在肝脂肪变性发生发展过程中的作用。整体实验设计：检测对照组（Alkbh1 f/f）与AKO小鼠肝细胞之间的差异表达基因。