Microbial Metagenome, Metatranscriptome & Phylogenetic Libraries. marine metagenome

Oxygen depleted water columns (ODWCs) appear to be expanding in response to global climate change. This alters trophic structure, compresses habitat and modifies geochemical cycles of major elements. Oxygen depletion can vary in intensity and duration from seasonal hypoxia to permanent anoxia. The focus of this study is a classic example of the anoxic end-member, the Cariaco Basin. The overall goal is to examine how microbial functional potential (metagenomic), activity (metatranscriptomic), taxonomic diversity (based on SSU rRNA) and the ecological/geochemical consequences (in terms of measured rates of key processes) relate along vertical oxygen/geochemical gradients and between seasons in the Cariaco Basin. This will reveal relationships between expression of particular sets of genes, environmental differences in nutrients, energy substrates and oxidant availabilities.缺氧水柱（Oxygen depleted water columns, ODWCs）正随全球气候变化呈现扩张态势。该现象会改变水体营养结构、压缩生物栖息地，并扰动主要元素的生物地球化学循环。氧气耗竭的强度与持续时间存在显著差异，可覆盖从季节性低氧到永久性缺氧的全谱系范围。本研究以永久性缺氧端元的典型案例——卡里科海盆（Cariaco Basin）为研究对象，总体目标是探究：在卡里科海盆中，微生物功能潜力（宏基因组学）、代谢活性（宏转录组学）、分类多样性（基于小亚基核糖体RNA，SSU rRNA），以及生态与生物地球化学后果（以关键过程的实测速率为量化指标），如何沿垂直方向的氧气/生物地球化学梯度发生变化，并随季节产生差异。本研究将揭示特定基因集的表达、营养盐与能源底物的环境差异，以及氧化剂可获得性三者间的内在关联。