Screen for Ferry-associated RNAs

Goal: Identification of RNAs that are associated with the novel Ferry complex Methods: We performed a GST pulldown experiment, where GST-Ferry and GST were incubated with a HEK 293 cell lysate and extensively washed prior to elution. Subsequently, RNA was extracted from the elution and sequenced. Results: We identifed around 17 588 different transcripts and found a set of 252 mRNAs that are specifically enriched in the GST-Ferry sample. Conclusions: The Ferry complex seems to interact with a specific subset of mRNAs mRNA extraction from a GST pulldown experiment using GST and GST-Ferry, incubated with HEK 293 lysate, in triplicates.研究目标：鉴定与新型Ferry复合物（Ferry complex）相关的RNA。 实验方法：本研究开展GST pull-down实验：将GST-Ferry融合蛋白与GST蛋白分别与HEK 293细胞裂解液共孵育，经充分洗涤后完成洗脱操作，随后从洗脱产物中提取RNA并进行测序。 实验结果：共鉴定到约17588种不同的转录本，同时发现有252个信使RNA（mRNA）在GST-Ferry组样品中特异性富集。 研究结论：Ferry复合物似乎可与特定子集的信使RNA发生相互作用。本次实验采用GST与GST-Ferry蛋白分别与HEK 293细胞裂解液共孵育的实验体系，并设置了三次生物学重复。