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Tara Pacific metabarcoding sequencing (16S, 18S, ITS2) reference & replication tables version 1

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Zenodo2023-06-05 更新2026-05-25 收录
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The tables in this dataset associate metabarcoding sequencing files generated by Genoscope (i.e. fastq.gz files with informative filename structures; with sequencing file pairs associated to unique 'readset' identifiers; housed on their FTP server at www.genoscope.cns.fr/sadc/tarapacific/METABARCODING; with the 'METAB' sequence strategy identifier as part of the sequencing file name) with their associated 'sample-id_source' identifier; as detailed in the 'TARA-PACIFIC_samples-provenance' file part of the 'Tara Pacific samples provenance and environmental context' Zenodo publication (DOI: 10.5281/zenodo.4068292) part of the 'tarapacific' Zenodo community). The main purpose of these tables is to act as a reference to identify samples (i.e., with a single 'sample-id_source') for which sequencing replication exists, per primer set (i.e., more than one set of fastq.gz sequencing files were generated, pre primer set). In the case of replication, these tables classify the replication into three classes color coded as green (same DNA extraction, same PCR, same sequencing run, different sequencing lane), yellow (same DNA extraction, same PCR, different sequencing run) and red (different DNA extraction and/or different PCR). It should be noted that in the vast majority of cases a 'sample-id_source' associates to only one readset per primer set. For a full description of the dataset, please see the included README.

本数据集内的表格将由Genoscope(法国国家基因测序中心)生成的metabarcoding(元条形码测序)测序文件——即文件名结构包含关键信息的fastq.gz文件,测序文件配对对应唯一的readset(测序数据集标识),存储于其FTP服务器www.genoscope.cns.fr/sadc/tarapacific/METABARCODING中,且测序文件名包含"METAB"序列策略标识符——与其关联的"sample-id_source"标识符进行关联。该关联关系详见"Tara Pacific样本溯源与环境背景"Zenodo出版物(DOI: 10.5281/zenodo.4068292)中的"TARA-PACIFIC_samples-provenance"文件部分,该出版物隶属于"tarapacific"Zenodo社区。本数据集表格的核心用途是作为参考依据,用于识别每个primer set(引物组)下存在测序重复的样本(即单个"sample-id_source"对应多套fastq.gz测序文件)。针对存在测序重复的样本,表格将其分为三类并以颜色编码区分:绿色组(同一DNA提取流程、同一PCR扩增反应、同一测序运行批次、不同测序泳道)、黄色组(同一DNA提取流程、同一PCR扩增反应、不同测序运行批次)、红色组(不同DNA提取流程和/或不同PCR扩增反应)。需特别说明,绝大多数情况下,单个"sample-id_source"在每个引物组下仅对应一个readset。若需完整了解本数据集的详细说明,请参阅附带的README文件。

提供机构:
Zenodo
创建时间:
2020-11-09
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