NEON: Directions and resources for long-term monitoring in soil microbial ecology

The EMP_Pilot represents a subset of soil samples from the National Ecological Observatory Network (NEON) microbe prototype project. The EMP_Pilot was designed as a cross-facility comparison to quantify variation in sample processing and outputs from two sequencing facilities (Argonne National Labs, Lawrence Berkeley Labs) for quality assurance. The twelve soil samples that comprise the EMP-Pilot were collected in 2009 from four NEON core domain sites in Alaska, Florida,Utah, and Hawaii and represent gradients in ecosystem and soil properties. Information regarding the NEON Domain sites can be found at www.neoninc.org/science/domains. In 2013, soil biochemical, phospholipid fatty acid (PLFA), and targeted gene sequence data associated with the EMP-Pilot samples will be available through NEON's data sharing system.EMP_Pilot 是国家生态观测站网络（National Ecological Observatory Network, NEON）微生物原型项目的土壤样本子集。该数据集被设计用于跨测序机构比对研究，以量化阿贡国家实验室（Argonne National Labs）与劳伦斯伯克利国家实验室（Lawrence Berkeley Labs）两家测序机构在样本处理流程及产出结果间的差异，进而开展质量管控。构成EMP_Pilot的12份土壤样本于2009年采集自阿拉斯加、佛罗里达、犹他州及夏威夷的4个NEON核心区域站点，涵盖了不同生态系统与土壤属性的梯度变化。有关NEON核心区域站点的详细信息，可通过网址www.neoninc.org/science/domains查询获取。2013年，与EMP_Pilot样本关联的土壤生化数据、磷脂脂肪酸（phospholipid fatty acid, PLFA）数据及靶向基因测序数据，将通过NEON的数据共享系统对外开放。