GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution. GLORI for absolute quantification of transcriptome-wide m6A at single-base resolution

We developed a quantitative method called GLORI to investigate m6A methylation in the mammalian transcriptome at single-base resolution. Overall design: We provided a detailed description of the specific workflow and intricate details of the GLORI technique.Also, we conducted a detailed evaluation of the performance of GLORI.本研究开发了一种名为GLORI的定量方法，用于在单碱基分辨率水平下研究哺乳动物转录组中的N6-甲基腺嘌呤（m⁶A）甲基化修饰。实验整体设计：我们详细阐述了GLORI技术的具体实验流程与精细技术细节，此外还对GLORI的性能开展了全面细致的评估。