Datesets for "Single-cell lipidomics enabled by dual-polarity ionization and ion mobility-mass spectrometry imaging"

Single-cell (SC) analysis provides unique insight into individual cell dynamics and cell-to-cell heterogeneity. Here, we utilize trapped ion mobility separation coupled with dual-polarity ionization mass spectrometry imaging (MSI) to enable high throughput in situ profiling of SC lipidome. Multimodal SC imaging, in which dual-polarity-mode MSI is used to perform serial data acquisition runs on individual cells, significantly enhanced SC lipidome coverage. High spatial resolution SC-MSI identifies both inter- and intra-cellular lipid heterogeneity; this heterogeneity is further explicated by Uniform Manifold Approximation and Projection and machine learning-driven classifications. We characterize SC lipidome alteration in response to stearoyl-CoA desaturase 1 (SCD1) inhibition and, additionally, identify layer-specific lipid distribution patterns in mouse cerebellar cortex. This integrated multimodal SC-MSI technology enables high-resolution spatial mapping of intracellular and cell-to-cell lipidome heterogeneity, SC lipidome remodeling induced by pharmacological intervention, and region-specific lipid diversity within tissue.单细胞（Single-cell, SC）分析能够为单个细胞的动态变化与细胞间异质性提供独特的研究视角。本研究中，我们将捕获离子迁移分离与双极性电离质谱成像（dual-polarity ionization mass spectrometry imaging, MSI）联用，实现单细胞脂质组的高通量原位表征。在多模态单细胞成像中，采用双极性模式质谱成像对单个细胞进行连续数据采集，显著提升了单细胞脂质组的覆盖范围。高空间分辨率的单细胞质谱成像可同时识别细胞间与细胞内的脂质异质性；该异质性可通过统一流形近似与投影（Uniform Manifold Approximation and Projection）以及机器学习驱动的分类方法进一步解析。我们对硬脂酰辅酶A去饱和酶1（stearoyl-CoA desaturase 1, SCD1）抑制诱导的单细胞脂质组改变进行了表征，此外还在小鼠小脑皮层中鉴定出了层特异性脂质分布模式。这一集成化多模态单细胞质谱成像技术，可实现细胞内与细胞间脂质组异质性的高分辨率空间定位、药物干预诱导的单细胞脂质组重塑，以及组织内区域特异性脂质多样性的分析。