RNA-seq profiling of malaria pre-exposed but resting effector memory ?d T cells

Plasmodium chabaudi infected mice were drug-treated on day 14 post-infection with an i.p. injection of CQ (10mg/kg) and pyrimethamine (10mg/kg) followed by CQ (6 mg/ml) and pyrimethamine (70 mg/ml) containing water for 5 days. Spleens were removed 12 weeks after completion of drug treatment. Spleens from drug-treated naive mice were used as controls. Overall design: Splenocytes were obtained and cells were surface stained ex vivo to identify CD62L-CD44+?d T cells (EM). These EM ?d T cells were FACS sorted and total RNA was isolated followed by ClonTech RNA sequencing.