RNA-seq profiling of malaria pre-exposed but resting effector memory ?d T cells

Plasmodium chabaudi infected mice were drug-treated on day 14 post-infection with an i.p. injection of CQ (10mg/kg) and pyrimethamine (10mg/kg) followed by CQ (6 mg/ml) and pyrimethamine (70 mg/ml) containing water for 5 days. Spleens were removed 12 weeks after completion of drug treatment. Spleens from drug-treated naive mice were used as controls. Overall design: Splenocytes were obtained and cells were surface stained ex vivo to identify CD62L-CD44+?d T cells (EM). These EM ?d T cells were FACS sorted and total RNA was isolated followed by ClonTech RNA sequencing.本数据集以感染查氏疟原虫（Plasmodium chabaudi）的小鼠为研究对象：于感染后第14天通过腹腔（intraperitoneal, i.p.）注射给予氯喹（Chloroquine, CQ，10mg/kg）与乙胺嘧啶（pyrimethamine，10mg/kg），随后让小鼠饮用含6mg/ml氯喹及70mg/ml乙胺嘧啶的饮水，持续5天。给药结束12周后摘取小鼠脾脏；以经相同药物处理的未感染（naive）小鼠脾脏作为对照。实验设计概述：离体分离脾细胞后，通过离体表面染色鉴定CD62L⁻CD44⁺ γδ T细胞（即效应记忆（Effector Memory, EM）表型γδ T细胞），经荧光激活细胞分选（fluorescence-activated cell sorting, FACS）纯化该类细胞，提取总RNA后采用ClonTech平台开展RNA测序。