16S ribosomal RNA gene of mice

The V3-V4 region of the 16S ribosomal RNA gene was targeted for amplicon sequencing using sequence-specific primers (341F: CCTAYGGGRBGCASCAG, 806R: GGACTACNNGGGTATCTAAT) with attached Illumina adapter overhang sequences.

本实验以16S核糖体RNA基因（16S ribosomal RNA gene）的V3-V4区域为靶标，采用带有附加Illumina衔接子突出序列的序列特异性引物（341F: CCTAYGGGRBGCASCAG、806R: GGACTACNNGGGTATCTAAT）开展扩增子测序。