Unprocessed and uncropped Western blot images [Pharmacol. Res. (2021) 166:105474]

In our previous study [Cheng et al., Pharmacological Research (2021) 166:105474] , we demonstrated that ROS scavenging by the use of Tiron partially attenuated the DNA damaging effects of BIRC5 down-regulation in MCF7 and MDA-MB-231 cells, as shown by the results of the Western blot analysis (Fig. 3E). Despite the ACTA1 blots (i.e. the blot images) used for the "MCF7 and MDA-MB-231 panels" are completely different and without manipulation; however, these blots "on certain degree" look a bit similar to each other.

In order to avoid creating confusion to the reader, we here provide the Raw (unprocessed and uncropped, and with a series of different exposure time) images in order to support the authenticity of the images published (Fig. 3E) in the article. On the images, the first 4 lanes belong to MCF7 and the next 4 lanes belong to MDA-MB-231. Out of these "4 lanes", only the first 3 lanes were used (i.e. Lane 1, 2, 3 and 5, 6, 7) for the figure.

在本团队此前的研究[Cheng等，《Pharmacological Research》（2021），166卷：105474]中，我们证实通过使用Tiron清除活性氧（Reactive Oxygen Species，ROS），可部分减轻BIRC5基因下调对MCF7和MDA-MB-231细胞引发的DNA损伤效应，该结论经由蛋白质免疫印迹（Western blot）分析验证（图3E）。尽管用于"MCF7与MDA-MB-231子图"的肌动蛋白α1（ACTA1）免疫印迹图像完全不同且未经过任何编辑处理，但这些印迹在一定程度上仍存在视觉相似性。
为避免对读者造成困惑，我们特此提供原始（未处理、未裁剪，且包含一系列不同曝光时长）的图像，以佐证本文刊发的图3E中图像的真实性。每张图像中，前4个泳道对应MCF7细胞，后续4个泳道对应MDA-MB-231细胞。在两组各4个泳道中，仅各取前3个泳道用于该图制作，即泳道1、2、3与泳道5、6、7。