RNA-Seq Arabidopsis RKD

In this study, in order to determine the regulatory control of RKD transcription factors on their downstream genes and characterize the gene network involved in the regulation of female gametophyte development, we performed a RNA-Seq analysis in ovules at the last stage of female gametophyte development, where all cell types are well established. To avoid possible amplification bias, non-amplified RNA of precisely staged WT and rkd mutant ovules was used for deep sequencing. Transcriptome analysis revealed changes in the expression of several gametophyte-specific gene families, ovule development-specific genes, and identified pleiotropic effects on phytohormone pathways.