RNA-Seq Arabidopsis RKD

In this study, in order to determine the regulatory control of RKD transcription factors on their downstream genes and characterize the gene network involved in the regulation of female gametophyte development, we performed a RNA-Seq analysis in ovules at the last stage of female gametophyte development, where all cell types are well established. To avoid possible amplification bias, non-amplified RNA of precisely staged WT and rkd mutant ovules was used for deep sequencing. Transcriptome analysis revealed changes in the expression of several gametophyte-specific gene families, ovule development-specific genes, and identified pleiotropic effects on phytohormone pathways.本研究旨在明确RKD转录因子对下游靶基因的调控作用，并解析参与雌配子体发育调控的基因网络，我们选取雌配子体发育最终阶段（此时所有细胞类型均已完全建立）的胚珠开展RNA测序（RNA-Seq）分析。为规避潜在的扩增偏倚，本实验采用精准分期的野生型（WT）与rkd突变体胚珠的非扩增RNA进行深度测序。转录组分析结果显示，多个配子体特异性基因家族、胚珠发育特异性基因的表达水平发生显著变化，同时鉴定出该调控过程对植物激素信号通路存在多效性影响。