DREAM in pain mechanisms in the trigeminal ganglia. Mus musculus

Expression of DREAM in dorsal root ganglia and spinal cord is related to endogenous control mechanisms of acute and chronic pain. In primary sensory trigeminal neurons high levels of endogenous DREAM protein are preferentially localized in the nucleus, suggesting a major transcriptional role. Here, we show that DREAM participates in the control of trigeminal pain perception through the regulation of prodynorphin and BDNF. Furthermore, genome-wide analysis of trigeminal neurons in daDREAM transgenic mice revealed that cathepsin L (CTSL) and the monoglyceride lipase (MGLL) are new DREAM downstream targets and have a role in the regulation of trigeminal nociception. Overall design: Four biological replicates were independently hybridized to Affymetrix chips (GeneChip Mouse Genome 430 2.0 Array) for each sample type.DREAM蛋白（DREAM）在背根神经节（dorsal root ganglia）与脊髓中的表达与急性和慢性疼痛的内源性调控机制密切相关。在初级感觉三叉神经元中，高水平的内源性DREAM蛋白优先定位于细胞核内，提示其主要发挥转录调控功能。本研究证实，DREAM通过调控强啡肽原（prodynorphin）与脑源性神经营养因子（Brain-Derived Neurotrophic Factor, BDNF）参与三叉神经痛觉感知的调控。此外，对daDREAM转基因小鼠三叉神经元开展的全基因组分析显示，组织蛋白酶L（cathepsin L, CTSL）与单酰甘油脂肪酶（monoglyceride lipase, MGLL）是新的DREAM下游靶基因，且参与三叉神经痛觉感知的调控。 整体实验设计：针对每种样本类型，设置四个生物学重复，分别独立使用Affymetrix芯片（GeneChip Mouse Genome 430 2.0 阵列）进行杂交实验。