Data_Sheet_1_Effects of Selenomethionine on Cell Viability, Selenoprotein Expression and Antioxidant Function in Porcine Mammary Epithelial Cells.XLSX

This study investigated the effects of selenomethionine (Se-Met) on the cell viability, selenoprotein expression, and antioxidant function of porcine mammary epithelial cells (pMECs) to reveal the underlying molecular mechanism of Se-Met on the lactation performance and antioxidant capacity of sows in vitro. The pMECs were used as an in vitro model and were treated with various concentrations of Se-Met (0, 0.5, 1, 2, and 4 μM). Cells were analyzed for cell viability, selenoprotein transcriptome, selenoprotein expression, and antioxidant enzyme activities. The results showed that, with increasing Se-Met concentrations, cell viability first increased and then decreased at 24, 48, or 72 h posttreatment with maximum values at 0.5-μM Se-Met. As the Se-Met concentrations increased, the mRNA expression of 17 selenoproteins first upregulated and then downregulated, with maximum values at 0.5-μM Se-Met. The 17 selenoproteins included SEPHS2, SELENOP, GPX1, GPX2, GPX3, GPX6, TXNRD1, SELENOK, SELENOW, DIO1, DIO2, DIO3, SELENOF, SELENOS, SELENOH, SELENOI, and SELENOT. Additionally, the protein expression levels of SEPHS2, SELENOP, GPX1, and TXNRD1 and the activities of glutathione peroxidase and thioredoxin were highest at 0.5-μM Se-Met. In conclusion, 0.5-μM Se-Met promotes cell viability partially by improving selenoprotein expression and antioxidant function in pMECs, which provides evidence for the potential ability of Se-Met to improve mammary gland health in sows.

本研究以硒代蛋氨酸（selenomethionine，Se-Met）为干预对象，探究其对猪乳腺上皮细胞（porcine mammary epithelial cells，pMECs）的细胞活力、硒蛋白表达及抗氧化功能的影响，旨在体外揭示Se-Met影响母猪泌乳性能与抗氧化能力的潜在分子机制。本研究以pMECs作为体外模型，采用0、0.5、1、2、4 μM的不同浓度Se-Met进行处理，随后从细胞活力、硒蛋白转录组、硒蛋白表达及抗氧化酶活性四个维度对细胞进行检测分析。结果显示，在处理后24、48或72小时，随着Se-Met浓度升高，细胞活力呈先上升后下降的趋势，且在0.5 μM Se-Met处理组达到峰值。随着Se-Met浓度增加，17种硒蛋白的mRNA表达量先上调后下调，同样在0.5 μM浓度组达到最大值，这17种硒蛋白包括SEPHS2、SELENOP、GPX1、GPX2、GPX3、GPX6、TXNRD1、SELENOK、SELENOW、DIO1、DIO2、DIO3、SELENOF、SELENOS、SELENOH、SELENOI及SELENOT。此外，SEPHS2、SELENOP、GPX1与TXNRD1的蛋白表达水平，以及谷胱甘肽过氧化物酶、硫氧还蛋白的活性，均在0.5 μM Se-Met处理组达到最高。综上，0.5 μM Se-Met可通过提升pMECs的硒蛋白表达与抗氧化功能，部分促进细胞活力，该研究为Se-Met改善母猪乳腺健康的潜在能力提供了实验依据。