Separable roles for RNAi in regulation of transposable elements and viability in the fission yeast Schizosaccharomyces japonicus [ChIP-Seq]. Separable roles for RNAi in regulation of transposable elements and viability in the fission yeast Schizosaccharomyces japonicus [ChIP-Seq]

ChIP-seq was performed to identify differences in H3K9me2 profiles between wild type and Dcr1 deficent cells in Schizosaccharomyces japonicus. Overall design: Two biological replicates of ChIP-Seq profiles in WT and dcr1D, using antibodies against total histone H3 and histone H3K9 dimethylation

本研究通过染色质免疫共沉淀测序（ChIP-seq），旨在鉴定日本粟酒裂殖酵母（Schizosaccharomyces japonicus）内野生型与Dcr1缺陷型细胞的组蛋白H3赖氨酸9二甲基化（H3K9me2）修饰谱差异。实验设计概述：针对野生型（WT）与dcr1D菌株，各设置两组生物学重复的ChIP-seq分析，所用抗体分别靶向总组蛋白H3与组蛋白H3赖氨酸9二甲基化。