Exploring Fur regulon in Escherichia coli. Exploring Fur regulon in Escherichia coli

To get a high resolution understanding of the effect of Fur on global gene expression, we compared by high-resolution RNAseq the transcriptomes of a wild-type E. coli K-12 strain and its Fur deletion derivative grown in minimal medium with or without supplementation of iron. Three independent total RNA extraction and RNAseq assays were performed for each strain in each condition. Overall design: Examination of the gene expression when E. coli NCM3722 grew in sorbitol carbon sources with iron or not, as well as its fur - mutant (CY405).为实现铁摄取调控因子（Fur）对全局基因表达影响的高分辨率解析，我们借助高分辨率RNA测序（RNAseq）技术，对比了在添加或不添加铁补充剂的基本培养基中培养的野生型大肠杆菌（E. coli）K-12菌株及其Fur缺失衍生菌株的转录组。针对每种培养条件下的每株菌株，我们均开展了三次独立的总RNA提取与RNA测序实验。整体实验设计：考察大肠杆菌NCM3722在以山梨醇为碳源且添加/不添加铁的培养基中生长时，及其fur缺失突变株（CY405）的基因表达情况。