Contribution of 6mer seed toxicity to HIV-1 induced cytotoxicity [DISE-36]

Analysis of RISC bound short (s)RNAs in cells infected with HIV-1 reveals a contribution of 6mer seed toxicity to HIV-1 induced cytopathicity Overall design: Ago protein associated short RNAs were pulled down using a GW182 peptide and subjected to smRNA Seq对HIV-1感染细胞中与RNA诱导沉默复合物（RNA-induced silencing complex, RISC）结合的短链RNA（sRNAs）的分析揭示了6聚体种子毒性对HIV-1诱导的细胞病变效应的贡献。整体实验设计：采用GW182肽段亲和捕获与Argonaute（Ago）蛋白结合的短链RNA，并进行smRNA Seq。