Anna Baccei, Susan Lancelle (2011) CIL:12598, Canis lupus familiaris, epithelial cell. CIL. Dataset

Parallel stress fibers on the ventral face of an MDCK cell, just inside the plasma membrane. This transmission electron micrograph of a flat-embedded MDCK cell was taken 60-70nm from the interface between the cell and the coverslip on which it was grown and fixed. The cells were cultured on Thermanox® coverslips, then chemically fixed with glutaraldehyde and osmium tetroxide before being stained en bloc with uranyl acetate. The cells were infiltrated with Spurr's® resin, placed under a vacuum for fifteen minutes, and then polymerized at 70° C. The coverslips were peeled away from the hardened resin wafers, which were then cut and thin-sectioned with a diamond knife. The 65 nm sections were placed on grids and post-stained with uranyl acetate and lead citrate. They were imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80kV. Dark spots at somewhat regular intervals along the stress fibers are likely to be focal adhesion sites. Scale bar = 1µm.

在MDCK细胞腹面靠近质膜处的平行应力纤维。该平展嵌入的MDCK细胞透射电子显微镜图像是在细胞与生长并固定的盖玻片之间界面60-70纳米处拍摄的。细胞培养于Thermanox®盖玻片上，随后用戊二醛和四氧化锇进行化学固定，并整体用醋酸铀染色。细胞被Spurr's®树脂渗透，在真空条件下放置十五分钟，然后在70°C下聚合。将盖玻片从硬化的树脂基片上剥离，随后用钻石刀切割并制成薄片。65纳米的切片放置在网格上，并经醋酸铀和柠檬酸铅后染色。它们使用Philips CM 100透射电子显微镜，在80kV的加速电压下进行成像。应力纤维沿一定规律间隔出现的暗点很可能是焦点粘附位点。比例尺=1微米。