SH1043296.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为界定物种边界、开展物种识别、进行学术交流以及处理基于DNA的物种假说（Species Hypotheses，简称SH）提供了一套统一的方法。国际核苷酸序列数据库收录的所有真菌内转录间隔区（Internal Transcribed Spacer，简称ITS）序列，均通过应用一组动态距离阈值（范围约0.5%至3.0%）被聚类至近似物种水平的集群。所有物种假说都会被赋予一个唯一且稳定的名称，格式为数字对象唯一标识符（Digital Object Identifier，简称DOI）；其分类学与生态学注释信息，将通过分布式、基于网页的第三方注释协作项目完成验证。每个SH都会尽可能匹配对应的分类单元名称及其所属分类层级（如门、纲、目等），关联过程会综合考量该SH下所有序列的鉴定结果。研究人员将选取一条经自动或人工指定的序列作为对应SH的代表序列。上述代表序列已发布于（https://unite.ut.ee/repository.php），可供全球科研社群使用，例如用于本地序列相似性检索以及下一代测序分析流程。随着公共真菌ITS序列库规模的持续增长，该系统及其配套数据将自动完成更新。