SH1702491.10FU

UNITE provides a unified way for delimiting, identifying, communicating, and working with DNA-based Species Hypotheses (SH). All fungal ITS sequences in the international nucleotide sequence databases are clustered to approximately the species level by applying a set of dynamic distance values (<0.5 - 3.0%). All species hypotheses are given a unique, stable name in the form of a DOI, and their taxonomic and ecological annotations are verified through distributed, web-based third-party annotation efforts. SHs are connected to a taxon name and its classification as far as possible (phylum, class, order, etc.) by taking into account identifications for all sequences in the SH. An automatically or manually designated sequence is chosen to represent each such SH. These sequences are released (https://unite.ut.ee/repository.php) for use by the scientific community in, for example, local sequence similarity searches and next-generation sequencing analysis pipelines. The system and the data are updated automatically as the number of public fungal ITS sequences grows.

UNITE为界定、识别、交流以及开展基于DNA的物种假说（Species Hypotheses，简称SH）相关工作提供了统一的标准化方法。国际核酸序列数据库中的全部真菌ITS序列，通过应用一套动态距离阈值（0.5%~3.0%），被聚类至近似物种级别的聚类单元。所有物种假说均会被赋予一个以数字对象标识符（Digital Object Identifier，DOI）为形式的唯一且稳定的名称，其分类学与生态学注释信息将通过分布式网络第三方注释工作完成审核验证。系统会综合每个物种假说内所有序列的鉴定结果，尽可能将其与对应分类单元名称及其所属分类层级（门、纲、目等）进行关联。研究人员会选取一条经自动或人工指定的序列作为每一类物种假说的代表序列。此类代表序列已在https://unite.ut.ee/repository.php 公开发布，可供科研社区用于本地序列相似性搜索、下一代测序分析流程等科研场景。随着公开真菌ITS序列数量的持续增长，该系统与配套数据将自动完成更新。