Raw Plaque-2.0 infection scores of the PCL screen for IAV, imaged and analysed at UZH.

virus indicates virus genotype, the PCL was tested against, compoundIdentifier indicates the UZH identifier for blinded testing by UZH, setPlate is the subset plate A to D and replicate refers to the replicate 1 to 4, wellRow and wellColumn indicate the well and plate indicate the screening plate sequence number. The Plaque2.0-based infection scores are numberOfNuclei reporting the number of nuclei based on Hoechst staining, numberOfInfectedNuclei refers to the number of GFP reporter-based number of infected nuclei, infectionIndex is the ratio of numberOfInfectedNuclei to numberOfNuclei, the number of GFP reporter-based plaques is given by numberOfPlaques and totalVirusIntensity indicates total GFP reporter signal intensity.

virus指代本次PCL所针对的病毒基因型。化合物标识符（compoundIdentifier）指由苏黎世大学（UZH, University of Zurich）开展盲法测试时所用的UZH标识符；分组板位（setPlate）指代A至D的子板分组，重复实验（replicate）对应1至4号重复样本。孔行号（wellRow）与孔列号（wellColumn）用于标识具体孔位，筛选板序列号（plate）则代表该筛选板的序列编号。基于Plaque2.0的感染评分体系中，细胞核总数（numberOfNuclei）用于报告经赫斯特（Hoechst）染色得到的细胞核数量；感染细胞核总数（numberOfInfectedNuclei）指代基于绿色荧光蛋白（GFP, Green Fluorescent Protein）报告基因标记的感染细胞核数量。感染指数（infectionIndex）为感染细胞核总数与细胞核总数的比值；噬菌斑总数（numberOfPlaques）代表基于GFP报告基因标记的噬菌斑数量，病毒总信号强度（totalVirusIntensity）则指代GFP报告基因的总信号强度。