Additional file 6 of qRAT: an R-based stand-alone application for relative expression analysis of RT-qPCR data

Additional file 6. Output of qRAT $$\Delta$$ Δ Cq and $$\Delta \Delta$$ Δ Δ Cq methods using the SP dataset: Table of mean $$\Delta$$ Δ Cq, $$-\Delta$$ - Δ Cq and relative quantity (RQ) values, where values of the treatment sample (T) and control sample (C) are normalized to the reference gene and mean $$\Delta \Delta$$ Δ Δ Cq and fold change (FC) values, where T and C samples are normalized by both, the reference gene and the calibrator C.

附加文件6。基于SP数据集的qRAT ΔCq（delta Cq）与ΔΔCq（delta-delta Cq）分析方法输出结果：包含均值ΔCq（delta Cq）、-ΔCq（negative delta Cq）及相对定量（RQ）数值的表格，其中处理组样本（T）与对照组样本（C）的数值均经内参基因归一化；同时包含均值ΔΔCq（delta-delta Cq）与倍数变化（FC）数值的表格，其中T与C样本同时通过内参基因与校准对照组（C）完成归一化。