ChIP-Seq analysis of switchgrass transcriptome in response to single drought and combinations of drought and heat stress. ChIP-Seq analysis of switchgrass transcriptome in response to single drought and combinations of drought and heat stress

The combined heat and drought stress influence the plant growth and development. Switchgrass is an economically important crop due to the availability of high biomass with little water and nutrient requirements. Earlier reports suggested that switchgrass growth and yield highly influenced by heat and drought. The mechanism behind heat and drought stress is not fully understood in switchgrass. This study has undertaken to analyze the epigenetic modification using ChIP-Seq analysis with the activation histone mark H3K4me3. Conclusion: Our study provides the first epigenomic analysis of heat and drought response in switchgrass. This comprehensive resource will provide other epigenomic regulated information in non-model plant species. Overall design: Method: We conducted solely drought treatment in switchgrass plant Alamo AP13 by withholding water after 45 days of growing. For the combination of drought and heat effect, heat treatment (35 °C/25 °C day/night) was imposed after 72 h of the initiation of drought. Samples were collected at 0 h, 72 h, 96 h, 120 h, 144 h, and 168 h after treatment imposition, genomic DNAwas extracted, and ChIP-Seq conducted.

复合高温与干旱胁迫会影响植物的生长发育。柳枝稷（Switchgrass）是一类具有重要经济价值的作物，因其可在低水、低养分条件下积累较高生物量而被广泛应用。已有研究表明，高温与干旱胁迫会显著影响柳枝稷的生长与产量，但目前针对柳枝稷响应此类复合胁迫的分子机制尚未完全阐明。
本研究旨在通过针对组蛋白激活标记H3K4me3的染色质免疫共沉淀测序（ChIP-Seq）技术，分析柳枝稷的表观遗传修饰模式。
结论：本研究首次完成了柳枝稷响应高温与干旱胁迫的表观基因组分析。本研究生成的综合表观基因组资源，可为非模式植物物种的表观调控相关研究提供重要的数据支撑。
实验设计与方法：本研究以栽培品种Alamo AP13柳枝稷为材料，在其生长45天后停止供水，开展单一干旱胁迫处理。对于复合胁迫处理组，在干旱处理启动72小时后，施加昼/夜温度为35℃/25℃的高温胁迫。分别在胁迫处理启动后的0 h、72 h、96 h、120 h、144 h及168 h采集样品，提取基因组DNA并开展ChIP-Seq测序。