Expression of wild-type and mutated twk-16 constructs.

Summary of expression of wild-type and mutated twk-16 constructs in transgenic C. elegans lines. The total number of animals scored is in parentheses with YFP expressing animals shown as a percentage of the total under the corresponding regions of the nervous system. Lines were produced with PCR fusion constructs with the expression vector shown in Figure 2B. Lines are denoted as wild-type (WT) or mutated (Mut) followed by the size (bp) of the experimental sequences. The experimental sequences in WT195 to 5′3′Mut85 are diagramed (Figure 7A, 7B) and were as follows: WT195 with WT24 sequences; Mut195 with mutations of WT24 sequences; WT85 with WT24 sequences; Mut85 with mutations of WT24; 5′Mut 85 with mutations of 5′ 17 bp of WT85; 3′Mut85 with mutations of 3′ 15 bp of WT85; and 5′3′Mut85 with mutations of both 5′ 17 bp and 3′ 15 bp of WT85. The experimental sequences used in constructs WT53 to Mut8 are shown in Figure 5B.