Expression of wild-type and mutated twk-16 constructs.

Summary of expression of wild-type and mutated twk-16 constructs in transgenic C. elegans lines. The total number of animals scored is in parentheses with YFP expressing animals shown as a percentage of the total under the corresponding regions of the nervous system. Lines were produced with PCR fusion constructs with the expression vector shown in Figure 2B. Lines are denoted as wild-type (WT) or mutated (Mut) followed by the size (bp) of the experimental sequences. The experimental sequences in WT195 to 5′3′Mut85 are diagramed (Figure 7A, 7B) and were as follows: WT195 with WT24 sequences; Mut195 with mutations of WT24 sequences; WT85 with WT24 sequences; Mut85 with mutations of WT24; 5′Mut 85 with mutations of 5′ 17 bp of WT85; 3′Mut85 with mutations of 3′ 15 bp of WT85; and 5′3′Mut85 with mutations of both 5′ 17 bp and 3′ 15 bp of WT85. The experimental sequences used in constructs WT53 to Mut8 are shown in Figure 5B.转基因秀丽隐杆线虫品系中野生型与突变型twk-16构建体的表达概况总结。受试动物总计数以括号标注，表达黄色荧光蛋白（Yellow Fluorescent Protein，YFP）的动物占对应神经系统区域总样本的百分比已一并列出。所有品系均通过图2B所示表达载体的PCR融合构建体制备得到，品系命名规则为：以野生型（Wild-type，WT）或突变型（Mutated，Mut）为前缀，后接实验序列的长度（碱基对，base pair）。WT195至5′3′Mut85所使用的实验序列已在图7A、7B中予以图示，具体如下：WT195携带野生型WT24序列；Mut195为WT24序列的突变体；WT85携带野生型WT24序列；Mut85为WT24序列的突变体；5′Mut85为WT85的5′端17 bp序列发生突变的变体；3′Mut85为WT85的3′端15 bp序列发生突变的变体；5′3′Mut85则同时对WT85的5′端17 bp与3′端15 bp序列进行了突变。WT53至Mut8构建体所使用的实验序列详见图5B。