Activation of GPCR-GNAS-PKA signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity

To investigate the machanism by which MC1R represses the expression of a subset of IFNg reponsvie genes. We then performed ATAC seq and H3K27ac ChIP-seq to identify promoter and enhancer of MC1R-repressed IFNg reponsive genes. We designed B16F10 ATAC seq under 4 different conditions and B16F10 H3K27ac ChIP-seq under 2 different conditions.

为探究黑皮质素1受体（MC1R）抑制部分干扰素γ（IFN-γ）应答基因表达的分子机制，我们随后开展了转座酶可及性测序（ATAC-seq）与组蛋白H3赖氨酸27乙酰化染色质免疫共沉淀测序（H3K27ac ChIP-seq）实验，以鉴定受MC1R抑制的干扰素γ应答基因的启动子与增强子。本研究设置了4种不同培养条件的小鼠黑色素瘤细胞系B16F10的ATAC-seq实验组，以及2种不同培养条件的小鼠黑色素瘤细胞系B16F10的H3K27ac ChIP-seq实验组。