UK renal cancer samples genotyped on Illumina OmniExpress BeadChip

Renal cell carcinoma (RCC) cases comprised adult patients with histologically proven RCC were collected through two sources within the UK. First, 856 cases from SORCE, a MRC collection of surgically treated RCC cases ascertained through UK clinical oncology centres. Second, 189 RCC cases collected through the ICR and Royal Marsden NHS Hospitals Trust. Cases included 590 clear cell carcinomas (CCCs), 42 papillary carcinomas (PCs), 33 chromophobe carcinomas (CCs) and 19 mixed or other histological subtypes. DNA was extracted from EDTA-venous blood samples using the conventional methods and quantified using PicoGreen (Invitrogen). Cases were genotyped using the Human OmniExpress-12 BeadChip according to the manufacturer's recommendations (Illumina Inc, San Diego, CA, USA). After strict QC, 944 cases were retained. Data provided in plink format.

Controls used were data from the Wellcome Trust Case Control Consortium 2 (WTCCC2) 1958 birth cohort and the UK Blood Service Control Group (available as EGAS00000000028).EGA study EGAS00001002336

本数据集的肾细胞癌（Renal cell carcinoma, RCC）病例均为经组织学确诊的成年RCC患者，通过英国境内两类渠道收集：其一，856例病例来自SORCE队列——该队列为英国医学研究理事会（Medical Research Council, MRC）收录的经手术治疗的RCC病例，通过英国临床肿瘤学中心确认入组；其二，189例RCC病例通过英国癌症研究所（Institute of Cancer Research, ICR）与皇家马斯登NHS信托医院收集获得。
本数据集涵盖的病例包括590例透明细胞癌（clear cell carcinomas, CCCs）、42例乳头状癌（papillary carcinomas, PCs）、33例嫌色细胞癌（chromophobe carcinomas, CCs）以及19例混合型或其他组织学亚型病例。
研究人员采用常规方法从乙二胺四乙酸（Ethylenediaminetetraacetic acid, EDTA）抗凝静脉血样本中提取基因组DNA，并使用PicoGreen（Invitrogen公司）进行定量检测。按照美国加利福尼亚州圣地亚哥市Illumina公司的官方操作指南，使用Human OmniExpress-12 BeadChip芯片对所有病例进行基因分型。经过严格的质量控制（Quality Control, QC）后，最终保留944例病例，所有数据以PLINK格式提供。
对照数据来自英国惠康信托病例对照研究联盟2（Wellcome Trust Case Control Consortium 2, WTCCC2）的1958年出生队列，以及英国血液服务中心对照群组（数据集编号EGAS00000000028）。本研究对应的欧洲基因组档案（EGA）研究编号为EGAS00001002336。