Synergistic interaction between <i>itga3</i> and <i>lama5</i>.

For each experimental treatment, numbers of embryos seen in the 4 phenotypic classes (WT fin, single blister, multiple blisters and degenerate fins) were assessed at 48 hpf. 1:xx values indicate injected dilution of 1 mM stock of used morpholino. Combined injections of morpholinos were performed in two ways, either co-injection of a mixture of morpholinos at the given doses, or sequential injection of the two morpholinos separately. Independent controls were done for both methods and each treatment is presented with two sub-columns of numbers for co-injection (C) and sequential injection (S). Numbers of embryos within one of the four different phenotypic categories (rows 1–4) are given in percent (%), total numbers of evaluated embryos per experiment (n) in row 5.

针对每一项实验处理，我们在受精后48小时（hours post-fertilization，hpf）对4种鳍部表型类别——野生型鳍（WT fin）、单水泡型、多水泡型以及退化鳍——中的胚胎计数进行了统计。标注为1:xx的实验体系代表使用了1 mM母液稀释得到的注射用吗啉代寡核苷酸（morpholino）。吗啉代寡核苷酸的联合注射采用两种方式：一是将两种吗啉代寡核苷酸按指定剂量混合后共同注射，二是分别依次注射两种吗啉代寡核苷酸。针对这两种注射方式均设置了独立对照，且每种实验处理均配有两个数值子列，分别对应共同注射组（C）与依次注射组（S）的统计结果。4种不同表型类别的胚胎数量以百分比（%）形式呈现（对应第1至4行），每个实验中被评估的胚胎总数量（n）则列于第5行。