Tissue-specific imprinting and heterogeneity of natural killer cells and group 1 innate lymphoid cells

We report here the identification, by CITE-seq and scRNA-seq, of distinct NK cells, ILC1s and intermediate subsets across 4 tissues at steady state. We found that unlike NK cells, which had a signature well conserved between organs, ILC1s exhibited a great heterogeneity depending on the tissue microenvironment, at both the phenotypic and transcriptomic levels. Finally, we performed a multidimensional comparison of these cells across tissues and identified sets of genes expressed by each related subsets. Overall design: Single-cell RNA sequencing of murine sorted CD45+ Lin– NKp46+ NK1.1+ cells from small intestine lamina propria, liver, salivary gland and spleen using 10x Genomics.