Particle-associated bacterioplankton communities across the Red Sea

Pelagic particle-associated bacterioplankton play crucial roles in marine ecosystems, influencing biogeochemical cycling and ecosystem functioning. However, their diversity, composition, and dynamics remain poorly understood, particularly in unique environments such as the Red Sea. In this study, we employed eDNA metabarcoding to comprehensively characterise bacterioplankton communities associated with pelagic particles in a three-dimensional assessment spanning depths from the surface to up to 2300 m along the full length of the eastern Red Sea within the exclusive economic zone of the Kingdom of Saudi Arabia. Our results reveal a diverse assemblage of taxa, with Proteobacteria, Cyanobacteria and Planktomycetota being the dominant phyla. We identified pronounced spatial variability in community composition among five major Red Sea geographical regions with a third of all amplicon sequence variants being unique to the Southern Red Sea in contrast to a very homogenous distribution along ..., , , # Particle-associated bacterioplankton communities across the Red Sea

Dataset DOI: [10.5061/dryad.vt4b8gv1k](10.5061/dryad.vt4b8gv1k)

## Description of the data and file structure

Data for this manuscript was collected via filtering of seawater obtained by BottleNet sampling to retain environmental DNA, which then was extracted and eluted and sequenced, targeting the V3V4 region of the 16S SSU rRNA gene to describe bacterial communities. In addition, environmental parameters of the seawater were collected using a CTD. 

### Files and variables

#### File: clean\_BPBN.csv

##### Variables

* asv: obtained ASVs from processing raw sequencing fastq files using the DADA2 algorithm. 
* RSW 7 - RSW 282: distinct sample location, GPS, depth and environmental parameters provided in Supplementary File 1. 
* Kingdom, Phylum, Class, Order, Family, Genus, Species: bacterial taxonomy ranks. (NA: no valid measurement)

**Description:** ASV-to-sample matrix 

ZENODO files: NA in Supplementary File...,

浮游颗粒附着型浮游细菌（pelagic particle-associated bacterioplankton）在海洋生态系统中发挥关键作用，调控生物地球化学循环与生态系统功能。然而，其多样性、群落组成及动态特征仍鲜为人知，在红海这类特殊海洋环境中尤为如此。本研究采用环境DNA（environmental DNA）元条形码技术，对沙特阿拉伯王国专属经济区内东红海全流域开展从表层至2300米水深的三维采样评估，以此全面表征浮游颗粒附着型浮游细菌群落。研究结果显示群落类群组成丰富，变形菌门（Proteobacteria）、蓝细菌门（Cyanobacteria）与浮游真菌门（Planktomycetota）为优势菌门。我们发现红海五大主要地理区域间的群落组成存在显著空间异质性，三分之一的扩增子序列变异（amplicon sequence variant，ASV）仅存在于南红海，而沿...的分布则极为均质...

# 红海全域颗粒附着型浮游细菌群落

数据集DOI：[10.5061/dryad.vt4b8gv1k](10.5061/dryad.vt4b8gv1k)

## 数据与文件结构说明

本研究的样本数据通过以下流程获取：采用BottleNet采样系统采集海水，经过滤保留环境DNA，随后对DNA进行提取、洗脱并测序，靶向16S SSU rRNA基因的V3V4可变区以解析细菌群落组成。此外，通过温盐深仪（Conductivity-Temperature-Depth，CTD）采集海水环境参数。

### 文件与变量

#### 文件：clean_BPBN.csv

##### 变量

* asv：通过DADA2算法处理原始测序fastq文件得到的扩增子序列变异（amplicon sequence variant，ASV）。
* RSW 7至RSW 282：对应不同采样点位、GPS坐标、水深及环境参数，详见补充文件1。
* Kingdom（界）、Phylum（门）、Class（纲）、Order（目）、Family（科）、Genus（属）、Species（种）：细菌分类学层级。（NA：无有效测量值）

**文件说明：** 扩增子序列变异-样本矩阵

ZENODO文件：补充文件中标记为NA...