CIL:21775

Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA, CIL 21789) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2, CIL 21749, 21739, 21759, and 21773, respectively) has a different gene knocked down by RNAi. This is the set that were treated with mad2 RNAi. There are 10 images of each sample, for a total of 50 images. The images were acquired on a Zeiss Axiovert 200M microscope. A recommended use for these five image sets is to algorithmically compute the number of cells in each image. A tab-delimited text file containing this information can be found at the URL indicated in the attribution box. The recommended citation when using these images is: We used the Drosophila Kc167 1 image set (Carpenter, et al., Genome Biology, 2006) available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc).

本数据集包含5份经DNA染料赫斯特33342（Hoechst 33342）染色的黑腹果蝇（Drosophila melanogaster）Kc167细胞样本。其中最后一份样本标记为nodsRNA（CIL 21789），为野生型细胞；其余4份样本分别标记为48、340、Anillin及mad2，对应CIL编号依次为21749、21739、21759及21773，每份样本均通过RNA干扰（RNAi）技术敲低了不同的靶基因，其中标记为mad2的样本即采用mad2 RNAi进行基因敲低的实验组。每份样本包含10张显微图像，总计50张图像，所有图像均通过蔡司（Zeiss）Axiovert 200M显微镜采集。该数据集的推荐应用场景为通过算法自动计算每张图像中的细胞数量。包含细胞计数对应信息的制表符分隔文本文件可在来源标注框中给出的URL处获取。使用该图像集时的推荐引用格式为：我们使用了来自布罗德生物图像基准数据集集合（Broad Bioimage Benchmark Collection，网址：www.broad.mit.edu/bbbc）的黑腹果蝇Kc167图像集（Carpenter等，《基因组生物学》，2006年）。