The Tabulae Paralytica: Multimodal single-cell and spatial atlases of spinal cord injury

This submission is a dataset of two modalities, single-nucleus transcriptomics and single-nuclei spatial transcriptomics in the spinal cords of mice. The single-nuclei transcriptomics data is harvested and profiled using 10x Genomics Chromium Single Cell Kit Version. The single-nuclei spatial transcriptomics data is harvested and profiled using Visium Spatial Gene Expression. We used single-nucleus RNA-sequencing and spatial transcriptomics to profile the mid thoracic spinal cord of mice over various conditions spanning from age, spinal cord injury (SCI) severity, drug treatments, time after SCI, sex and injury models本提交数据集包含两种模态数据：小鼠脊髓的单细胞核转录组学（single-nucleus transcriptomics）与单细胞核空间转录组学（single-nuclei spatial transcriptomics）数据。其中单细胞核转录组学数据采用10x Genomics Chromium单细胞试剂盒（10x Genomics Chromium Single Cell Kit）版本完成样本获取与表达谱分析；单细胞核空间转录组学数据则借助Visium空间基因表达（Visium Spatial Gene Expression）技术进行样本采集与测序分析。本数据集构建团队通过单细胞核RNA测序（single-nucleus RNA-sequencing）与空间转录组学技术，针对小鼠胸段脊髓在多种实验条件下开展表达谱分析，所覆盖的实验变量包括年龄、脊髓损伤（spinal cord injury, SCI）严重程度、药物干预方案、损伤后时长、性别以及不同损伤模型。