Figure 7 - A549 (53BP1+PCNA) - Control Cells - Raw LIF

A549 Lung Adenocarcinoma cells were engineered to co-express a PCNA chromobody tagged with mNeonGreen (green) and a truncated version of 53BP1 tagged with mApple (red). Cells were synchronised with 1mM thymidine and pulsed with 5µg/ml cisplatin for 2 hr prior to release to enrich cells in early S phase. Cells were then imaged using a Leica SP8 confocal microscopy, using HyD detectors a WLL and 63X 1.4 NA objective. A 10-µm-thick z-stack (1 µm slice, 1AU) was taken every 30 min for 72 hr, with the maximum projection raw LIF and compressed MP4 files included here.<br>This data relates to Figure 7 of the published article:Rajal AG, Marzec KA, McCloy RA, Nobis M, Chin V, Hastings JF, Lai K, Kennerson M, Hughes WE, Vaghjiani V, Timpson P, Cain JE, Watkins DN, Croucher DR, Burgess A (2021) A non-genetic, cell cycle dependent mechanism of platinum resistance in lung adenocarcinoma. Elife 10:e65234. https://doi.org/10.7554/elife.65234

本数据集采用的A549肺腺癌细胞（A549 Lung Adenocarcinoma cells）经工程化改造，可共表达带有mNeonGreen（mNeonGreen）绿色标签的PCNA染色体纳米抗体（PCNA chromobody），以及带有mApple（mApple）红色标签的截短型p53结合蛋白1（53BP1）。细胞采用1mM胸苷（thymidine）进行细胞周期同步化处理，在解除同步化前以5µg/ml顺铂（cisplatin）脉冲处理2小时，以富集早期S期细胞。随后使用徕卡SP8共聚焦显微镜（Leica SP8 confocal microscopy）对细胞进行成像，配置HyD探测器（HyD detectors）、白光激光源（WLL）及63倍1.4数值孔径物镜（63X 1.4 NA objective）。以每30分钟1次的频率采集10μm厚的Z轴堆叠成像（z-stack）数据，每层切片厚度1μm，采用1艾里斑单位（1AU）的光圈参数，持续成像72小时；本数据集包含原始LIF格式（LIF）最大强度投影文件与压缩后的MP4格式（MP4）文件。
本数据集对应已发表论文的图7：Rajal AG, Marzec KA, McCloy RA, Nobis M, Chin V, Hastings JF, Lai K, Kennerson M, Hughes WE, Vaghjiani V, Timpson P, Cain JE, Watkins DN, Croucher DR, Burgess A (2021) 肺腺癌铂耐药性的非遗传、细胞周期依赖型机制. eLife 10:e65234. https://doi.org/10.7554/elife.65234