Chromosome-level genome assemblies of the migratory locust and the desert locust-6

A combination of three sequencing technologies including long-read sequencing (PacBio Sequel), optical mapping (Bionano Saphyr), and chromosome conformation capture technique based on proximity ligation (Hi-C) was applied to construct the chromosome-level genome assembly of the migratory locust (Locusta migratoria). Moreover, short-read DNA-seq (Illumina Data) both from female and male locusts was used to correct assembly errors. To predict protein-coding genes, we further generated short-read RNA-seq and PacBio ISO-seq cDNA data obtained from 11 different tissues and organs of the migrator locust. To track the chromosome dynamics of X chromosome within Orthoptera, we also generated a chromosome-level genome of the desert locust (Schistocerca gregaria) using Nanopore long-read sequencing and Illumina data with Hi-C scaffolding.

本研究结合长读长测序（long-read sequencing，PacBio Sequel）、光学图谱（optical mapping，Bionano Saphyr）以及基于邻位连接的染色体构象捕获技术（Hi-C）三种技术，用于构建飞蝗（Locusta migratoria）的染色体水平基因组组装结果。此外，本研究还利用取自雌性和雄性飞蝗的短读长DNA测序（short-read DNA-seq，Illumina数据）对组装错误进行校正。为预测蛋白质编码基因，我们进一步获取了取自该飞蝗11种不同组织与器官的短读长RNA测序（RNA-seq）数据以及PacBio ISO-seq cDNA测序数据。为追踪直翅目（Orthoptera）昆虫X染色体的染色体动态变化，本研究还采用纳米孔长读长测序（Nanopore long-read sequencing）结合Illumina数据与Hi-C挂载（Hi-C scaffolding）策略，构建了沙漠蝗（Schistocerca gregaria）的染色体水平基因组。