Additional file 1 of Transcriptional and protein structural characterization of homogentisate phytyltransferase genes in barley, wheat, and oat

Additional file 1: Supplementary Data S1. Sequence alignment and phylogenetic tree files. Supplementary Data S2. Retrieved transcriptome data for the target HPT genes. Supplementary Data S3. Primer sequences used in qRT-PCR. Supplementary Data S4. Putative promoter region sequences and cis-acting element prediction results. Supplementary Data S5. Natural selection output files. Supplementary Data S6. Generated protein models of HvHPT1 and HvHPT2 with docked substrates HGA and PDP in the optional conformation.

附加文件1：补充数据集S1，包含序列比对与系统发育树文件。
补充数据集S2：已获取的靶标HPT基因转录组数据。
补充数据集S3：实时定量反转录聚合酶链反应（qRT-PCR）所用引物序列。
补充数据集S4：推定启动子区域序列及顺式作用元件预测结果。
补充数据集S5：自然选择分析输出文件。
补充数据集S6：构建得到的HvHPT1与HvHPT2蛋白质模型，其中底物HGA与PDP以可选构象完成分子对接。