Transcriptional Effects of Melatonin of the Gut Commensal Bacterium Klebsiella aerogenes. Transcriptional Effects of Melatonin of the Gut Commensal Bacterium Klebsiella aerogenes

In this study, we implemented RNA sequencing to identify melatonin sensitive transcripts during culture maturation. This work demonstrates that majority of melatonin sensitive genes are growth stage specific. Melatonin caused differential gene expression of 81 transcripts during the exponential growth and 30 during early stationary phase. This indole molecule affects genes related to the biofilm formation, fimbria biogenesis, transcriptional regulators, carbohydrate transport and metabolism, phosphotransferase system (PTS), stress response, metal ion binding and transport. It is likely that differential expression of biofilm and fimbria related genes is responsible for differences in macrocolony area. Additionally, melatonin potentially helps Klebsiella aerogenes in host colonization. Overall design: K. aerogenes mRNA profiles of exponential cells grown with 0nM or 1nM melatonin and early stationary cells incubated with 0nM or 1nM melatonin

本研究通过RNA测序（RNA sequencing）技术，旨在筛选培养成熟过程中受褪黑素（melatonin）调控的转录本（transcripts）。本研究证实，绝大多数褪黑素敏感基因具有生长阶段特异性。在指数生长阶段，褪黑素可诱导81个转录本发生差异基因表达；在早期稳定生长期，则调控30个转录本的差异表达。该吲哚类分子可影响与生物被膜形成、菌毛生物发生、转录调控因子、碳水化合物转运与代谢、磷酸转移酶系统（PTS）、应激响应、金属离子结合与转运相关的基因表达。推测生物被膜与菌毛相关基因的差异表达，是导致宏观菌落面积产生差异的原因。此外，褪黑素可能助力产气克雷伯菌（Klebsiella aerogenes）在宿主内定殖。实验整体设计：对添加0nM或1nM褪黑素培养的指数生长期产气克雷伯菌细胞，以及添加0nM或1nM褪黑素孵育的早期稳定生长期产气克雷伯菌细胞，进行mRNA转录组分析。