RNASeq analysis of P. falciparum following exposure to xanthoquinodin A1. Plasmodium falciparum 3D7

RNA sequencing data was acquired in an effort to uncover more about the malaria parasite P. falciparum and the mode of action of peptaibol inhibitors. To this end, the in vitro parasite line 3D7 was synchronized and exposed in the trophozoite stage to an EC50 concentration of the inhibitor xanthoquionodin A1 I for 1 hour, or to a DMSO vehicle control. Total RNA was then extracted from parasite cellular material that had been separated from the host cell. A comparison of the expression levels of the messenger RNA and long non coding RNA was then performed between the treated and control samples. Multiple compounds tested alongside each other with a shared DMSO vehicle control which is published at BioProject: PRJNA1018932.

本数据集通过RNA测序（RNA sequencing）数据获取，旨在深入探究恶性疟原虫（Plasmodium falciparum，缩写P. falciparum）及肽醇类（peptaibol）抑制剂的作用模式。为此，研究人员对体外培养的疟原虫3D7株系进行同步化处理，并在滋养体（trophozoite）阶段将其暴露于抑制剂黄喹诺定A1 I（xanthoquionodin A1 I）的半数效应浓度（EC50）环境中处理1小时，同时设置二甲基亚砜（DMSO）溶剂对照。随后从与宿主细胞分离得到的疟原虫细胞材料中提取总RNA，对处理组与对照组样本中的信使RNA（messenger RNA, mRNA）及长链非编码RNA（long non-coding RNA, lncRNA）的表达水平开展对比分析。本研究同时测试了多种化合物并共用该DMSO溶剂对照，相关数据集已提交至BioProject数据库，登录编号为PRJNA1018932。