Diabetes microbiota study from a representative Saudi Population

Sample-specific index sequences, as well as sequences required for sequencing on Illumina instruments were incorporated into the aforementioned 16S gene segments by PCR. Libraries were sequenced on an Illumina MiSeq instrument using sequencing by synthesis.通过聚合酶链式反应（Polymerase Chain Reaction，PCR），将样本特异性索引序列以及Illumina测序仪测序所需的序列，整合至前述16S基因片段中。测序文库在Illumina MiSeq测序仪上采用边合成边测序（Sequencing by Synthesis）技术进行测序。