Exposure to lung-migrating helminth protects against murine SARS-CoV-2 infection through macrophage-dependent T cell activation. Exposure to lung-migrating helminth protects against murine SARS-CoV-2 infection through macrophage-dependent T cell activation

Helminth infection leads to lung remodelling. We used single cell ATAC sequencing (scRNA-seq) to analyze the changes in lung cells following exposure to N. brasiliensis at day 28 and day 45 post helminth infection Overall design: Single cell suspension of lungs cells was obtained from pooled samples from controls and helminth exposed mice. CD45+ immune cells were enriched in the pooled samples

蠕虫感染可诱导肺组织重塑。本研究采用单细胞ATAC测序（scRNA-seq），分析了蠕虫感染后第28天及第45天的巴西日圆线虫（Nippostrongylus brasiliensis，N. brasiliensis）暴露小鼠的肺细胞变化情况。
实验设计：从对照组及蠕虫感染暴露小鼠的混合样本中分离制备肺细胞单细胞悬液，并在混合样本中富集CD45阳性免疫细胞。