Derivation of porcine extra-embryonic endoderm cell lines reveals distinct signaling pathway and multipotency states. Derivation of porcine extra-embryonic endoderm cell lines reveals distinct signaling pathway and multipotency states

We detected the gene expression differences between porcine extra-embryonic endoderm cells and naïve like and primed porcine ESCs. Gene expression of porcine extra-embryonic endoderm cells was distinct with porcine naïve like and primed embryonic stem cells. Pearson correlation analysis confirmed that porcine naïve like and primed ESCs have stronger correlation than the correlation between either pXEN cells and porcine naïve like ESCs or primed ESCs. Porcine extra-embryonic endoderm cells have a distinct identity, clustering neither with porcine naïve like ESCs nor with primed ESCs. We confirmed that porcine extra-embryonic endoderm cells lack the expression of key pluripotency genes, such as Sox2, Pou5f1 and Klf4, but they expressed other pluripotency genes, such as Sall4, Lin28a and Klf5. Furthermore, porcine extra-embryonic endoderm cells robustly express Gata4, Gata6 and Sox17 as well as genes encoding ExEn-associated cell surface proteins or basement membrane components Pdgfra, Col4a2, Lama1, Lamb1, Sparc, Ihh, Hnf4a and Dab2, which is in contrast to porcine naïve like ESCs that express these genes at a low level or lack expression altogether Overall design: mRNA profiles of 3 porcine extra-embryonic endoderm cell lines, 2 naïve like embryonic stem cell lines and 2 primed embryonic stem cell lines in porcine.

本研究检测了猪胚外内胚层细胞（porcine extra-embryonic endoderm cells）与幼稚样猪胚胎干细胞（naïve-like porcine Embryonic Stem Cells, ESCs）及始发态猪胚胎干细胞之间的基因表达差异。猪胚外内胚层细胞的基因表达谱与幼稚样猪胚胎干细胞、始发态猪胚胎干细胞存在显著差异。皮尔逊相关分析证实，幼稚样猪胚胎干细胞与始发态猪胚胎干细胞之间的相关性，强于猪胚外内胚层细胞分别与幼稚样猪胚胎干细胞、始发态猪胚胎干细胞之间的相关性。猪胚外内胚层细胞具有独特的细胞身份特征，既不与幼稚样猪胚胎干细胞聚类，也不与始发态猪胚胎干细胞聚类。本研究证实，猪胚外内胚层细胞不表达关键多能性基因（如Sox2、Pou5f1及Klf4），但可表达其他多能性基因，如Sall4、Lin28a及Klf5。此外，猪胚外内胚层细胞可稳定高水平表达Gata4、Gata6、Sox17，以及编码胚外内胚层相关细胞表面蛋白或基底膜成分的基因（如Pdgfra、Col4a2、Lama1、Lamb1、Sparc、Ihh、Hnf4a及Dab2）；这与幼稚样猪胚胎干细胞形成鲜明对比，后者仅低水平表达这些基因，甚至完全不表达。实验整体设计：本研究涵盖3株猪胚外内胚层细胞系、2株幼稚样猪胚胎干细胞系及2株始发态猪胚胎干细胞系的mRNA表达谱分析。